ASCP ASCP-MLT Exam Details & Actual Exam Questions

  • Exam Code/Number: ASCP-MLT
  • Exam Name/Title: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Certification Provider: ASCP
  • Corresponding Certification: MLT(ASCP)
  • Exam Questions: 562
  • Updated On: Jul,10 2026
  • Certification Level: Technician

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Exam Questions

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ASCP ASCP-MLT Exam Overview:

Certification Vendor:American Society for Clinical Pathology Board of Certification (ASCP BOC)
Exam Name:Medical Laboratory Technician
Exam Number:MLT(ASCP)
Available Languages:English
Passing Score:Scaled score of ≥ 400 (on 100–999 scale, CAT scoring)
Real Exam Qty:Approximately 100
Certificate Validity Period:Certification maintenance required (completion of ongoing continuing education every 3 years)
Exam Price:Application fee (USD 235–250, varies by region and international discounts)
Exam Format:Computer Adaptive Testing (CAT), Multiple-choice questions (one best answer)
Exam Duration:150 minutes
Related Certifications:MLS(ASCP) Medical Laboratory Scientist
PBT(ASCP) Phlebotomy Technician
Sample Questions:ASCP ASCP-MLT Sample Questions
Exam Way:Computer-based testing delivered at Pearson VUE test centres
Pre Condition:Completion of accredited MLT program or equivalent education/training OR relevant clinical experience following ASCP BOC eligibility routes
Official Syllabus URL:https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT

ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Urinalysis and Other Body Fluids5–10%- Physical and chemical testing
- Microscopic analysis and disease correlation
Hematology20–25%- Physiology and disease states
- Laboratory testing and hemostasis
Laboratory Operations5–10%- Quality assessment and safety
- Instrumentation and laboratory mathematics
Microbiology15–20%- Virology and parasitology testing
- Bacteriology and mycology procedures
Chemistry20–25%- Carbohydrates, lipids, and enzymes
- Electrolytes, acid-base, and toxicology
Blood Banking15–20%- Transfusion practice and testing
- Blood products and components
- Blood group systems and immunology
Immunology5–10%- Principles of immunology
- Serologic procedures and immune diseases


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